A multiplex functional in vitro high throughput assay to measure compound effects on the inflammasome

Inflammasomes act as part of the innate immune response to induce inflammation upon recognition of pathogenic or damage signals. They are therefore key regulators of the inflammatory response. 

Nucleotide-binding oligomerization domain-like receptors family pyrin domain containing 3 (NLRP3) is one such inflammasome component that has been well characterised in neuroinflammation with links to chronic pain and neurodegenerative diseases, such as Parkinson’s and Alzheimer’s Disease, as well as a number of other diseases such as cancer and diabetes.

Domainex has established a robust activity assay for screening compounds for inhibition of NLRP3 inflammasome, which can be performed in THP-1 cells or human whole blood. 

Inflammasome assay method